◎ 제 271차 한림의학세미나 개최 ◎



▶ 일 시 : 2016. 11. 15(화) 16시 30분

▶ 장 소 : 대학원의학과 세미나실(의학관 5층 3506호실)

▶ 연 자 : 윤도영 교수(건국대학교 융합생명특성학과)

▶ 주 최 : 의과대학 의과학연구소 / 미생물학교실

▶ 강의제목 : Noncanonical function of a new inflammatory cytokine IL-32: 2005-2016

　IL-32 has been reported as NK 4 transcript that produced in T cell or NK cell and thought to be secreted because of the presence of signal sequence and the lack of transmembrane region, but its function has remained unclear. Recently, IL-32 has been redefined as a proinflammatory cytokine, and it is induced in epithelial cells and monocytes by IFN-γ stimulation in a time-dependent manner (IL-32: a new cytokine and inducer of TNFα. Immunity 22(1), 131, 2005).
Nine isoforms of IL-32 by alternative splicing have been reported (Biochimie, 2014), but functional differences among isoforms have not yet been elucidated. There are many uncertain and doubtful points regarding on IL-32: IL-32 is hardly detected in culture media; IL-32 has no signal sequence; IL-32 has too many isoforms; IL-32 isoforms are localized and interacted each other intracellularly: there is no IL-32 gene in mice and IL-32 receptor has not been reported yet.

In order to investigate the non-cannonical function of isoforms, we focused on the following areas:

1. Establishment of stable inflammatory and immune cells expressing new IL-32α, β, γ or θ isoform.
　1) Promonocytic myeloma THP-1, KG-1a as well as lymphoma cell Jurkat expressing IL-32 isoforms.
　2) Search for genes such as cytokines and cell surface expressing genes modulated by IL-32

2. Preparation of IL-32mAbs for Confocal microscope, Immunohistochemistry, Western blot, and ELISA systems. IL-32 mAbs were also prepared for the relational study with related diseases (J. Immunol Method 333: 38; Proc Natl Acad Sci USA 103(9): 3298). In order to identify IL-32BP and regulated proteins, Proteomics/Yeast two hybrid system were also applied. IL-32 Tg mice were also established in order to study the function of a new cytokine (Oncogene 30: 3345, 2011; Cell Death Dis. 4: e640, 2013)

3. Interaction network mapping among IL-32 isoforms
　1) Comprehensive analyses to reveal 81 interactions between nine IL-32 isoforms (Biochimie 2014 Jan 25).
　2) IL-32δ interacted with IL-32β and inhibited IL-32β-mediated IL-10 production (FEBS Lett. 587: 3776, 2014)

4. Functional analysis of IL-32 isoforms mediated via their interacting factors in the isoform expressing cell lines.
　1) Intracellular association of IL-32α with PKCε and STAT3 augments IL-6 production in THP-1 promonocytic cells. J Biol Chem 287(42): 35556-64, 2012
　2) IL-32β-mediated C/EBPα phosphorylation by PKCδ abrogates the inhibitory effect of C/EBPα on IL-10 production. J Biol Chem 288 (33): 23650, 2013
　3) More functional studies on differentiation of monocytic cells (Oncortaget, 2015; …).




▶ 학력:
1984.02: 서울대학교 응용생명화학(농화학) 학사
1988.02: KAIST 면역생화학 전공 석사
1994.08: KAIST 면역/생화학 전공 박사


▶ 경력:
2005.12.27 – 현재 건국대학교 융합생명특성학과 교수 세포생물/면역생화학
2013.10.07 – 2014.11.30 한국생명공학연구원 겸임연구원 WCI센터, 무급
2001.03.01 – 2005.12.26 한국생명공학연구원 책임연구원 연구책임자
1994.03.01 – 2001.02.28 한국생명공학연구원 선임연구원
1988.03.01 – 1994.02.28 한국생명공학연구원 연구원
1996.02.01 – 1997.11.30 Univ. Colorado Health Sciences Center Post-doc.fellow IL-1/IL-18
1995.10.15 – 1996.01.31 New England Medical Center, Tufts Univ Post-doc.fellow IL-1/IL-18
2004.09.01 – 2005.12.26 연합대학원(Univ. of Sci & Technol) 교수 출연연 연합대학원
2004.09.01 – 2005.12.26 한남대학교식품영양학과 겸임 교수
1999.08.30 – 2003.08.31 창원대학교보건생화학과 겸임 교수
1999.08.30 – 2000.02.29 충남대학교 대학원 강사 생체신호전달론
2001.03.16 – 2001.08.26 충남대학교 대학원 강사 생화학특론
2002.08.26 – 2003.02.28 충남대학교 대학원 강사 세포배양특론
2003.08.25 – 2004.02.29 충남대학교 대학원 강사 세포신호전달론
2006.12. – 2004.02.29 과기부 울트라 프로그램 암 연구 분과위원 과학기술정책연구원
2005.12. – 2008.12 PPAR Research Editorial Board 국제 전문학회지
2006.05 Animal Biotechnology & Experimental Medicine Editorial Board 국내 전문학회지
1988.03 한국생화학분자생물학회 종신회원
2007.3 – 2009.12 한국응용생명화학회 간사
2010.12 한국식품영양학회 정회원
2011.01 대한면역학회 학술위원





▶ 연구논문실적(2014~2016):

242. Kwon T, Bak Y, Park YH, Jang GB, Nam JS, Yoo JE, Park YN, Bak IS, Kim JM, Yoon DY, Yu DY (2016) Peroxiredoxin II is essential for maintaining stemness by redox regulation in liver cancer cells. Stem Cells. 2016 May; 34(5):1188-97. (co-corresponding)
241. Sae-Bom Kwon, Min-Je Kim, JinMo Yang, Hee-Pom Lee, JinTae Hong, Heon-Sang Jeong, Eun SukKim and Do-YoungYoon (2016) Cudrania tricuspidata stem (CTS) extracts induce apoptosis via the extrinsic pathway in SiHa cervical cancer cells. PLOS One 2016 Mar 9;11(3):e0150235.
240. Bak Y, Kwon T, Bak IS, Hong J, Yu DY, Yoon DY (2016) IL-32θ inhibits stemness and epithelial-mesenchymal transition of cancer stem cells via the STAT3 pathway in colon cancer. Oncotarget. Feb 9;7(6):7307-17.
239. Lee DH, Park MH, Hwang CJ, Hwang JY, Yoon HS, Yoon DY, Hong JT (2016) CCR5 deficiency increased susceptibility to lipopolysaccharide-induced acute renal injury. Arch Toxicol. 2016 May;90(5):1151-62.(co-corresponding)
238. Kwon T, Bak Y, Ham SY, Yu DY, Yoon DY (2016) A1E reduces stemness and self-renewal in HPV 16-positive cervical cancer stem cells. BMC Complement Altern Med. 2016 Feb 2;16(1):42
237. Park MH, Yoon DY, Ban JO, Kim DH, Lee DH, Song S, Kim Y, Han SB, Lee HP, Hong JT (2015) Decrease severity of collagen antibody and lipopolysacchride- induced arthritis in human IL-32β overexpressed transgenic mice. Oncotarget 6(36): 38566 - 38577 (2015 Nov 17) (co-1st author)
236. Kim MS, Kang JW, Jeon JS, Kim JK, Kim JW, Hong J, Yoon D (2015) IL-32θ gene expression in acute myeloid leukemia suppresses TNF-α production. Oncotarget 6(38):40747-61 (2015 Dec 1).
233. Sun Young Ham, Taeho Kwon, Yesol Bak, Jae-Hyuk Yu, Jintae Hong, Sang Kook Lee, Dae-Yeul Yu and Do-Young Yoon (2015) Mucin 1-mediated chemoresistance in lung cancer cells. Oncogenesis. 2016 Jan 18;5:e185.
230. Yun HM, Kim JA, Hwang CJ, Jin P, Baek MK, Lee JM, Hong JE, Lee SM, Han SB, Oh KW, Choi DY, Yoon DY, Hong JT (2015) Neuroinflammatory and Amyloidogenic Activities of IL-32β in Alzheimer's Disease. Mol Neurobiol. 52(1), 341-352
224. Dong Hun Lee, JiEun Hong, Hyung-Mun Yun, Chul Ju Hwang, JuHo Park, Sang Bae Han, Do Young Yoon and JinTae Hong (2015) Interleukin-32β ameliorates metabolic disorder and liver damage in mice fed high fat diet. Obesity 23(3) 615-622 (2015 March)
223. Hwang CJ, Yun HM, Jung YY, Lee DH, Yoon NY, Seo HO, Han JY, Oh KW, Choi DY, Han SB, Yoon DY, Hong JT (2015) Reducing Effect of IL-32α in the Development of Stroke Through Blocking of NF-κB, but Enhancement of STAT3 Pathways. Mol Neurobiol. 51(2): 648-660 (cocoresponding, April 2015)
222. Yun HM, Park KR, Kim EC, Han SB, Yoon DY, Hong JT (2015) IL-32α suppresses colorectal cancer development via TNFR1-mediated death signaling. Oncotarget. 2015 Apr 20;6(11):9061-72.
216. Man Sub Kim, Jeong-Woo Kang, Yun Sun Park, Dong Hun Lee, Yesol Bak, Taeho Kwon, Do-Young Yoon (2015) The modulating effect of ectopic expression of IL-32θ on the differentiation of monocyte into macrophage. Oncotarget 6(6): 4394-405
215. Park JS, Lee S, Jeong AL, Han S, Ka HI, Lim JS, Lee MS, Yoon DY, Lee JH, Yang Y (2015) Hypoxia-induced IL-32β increases glycolysis in breast cancer cells. Cancer Lett. 2015 Jan 28;356(2 Pt B):800-8.
213. Yun HM, Park MH, Kim DH, Ahn YJ, Park KR, Kim TM, Yun NY, Jung YS, Hwang DY, Yoon DY, Han SB, Hong JT (2014) Loss of presenilin 2 is associated with increased iPLA2 activity and lung tumor development. Oncogene 2014 Oct 30; 33(44): 5193 –200
212. Man Sub Kim, Jeong-Woo Kang, Dong Hun Lee, Yesol Bak, Yun Sun Park, Yong-Seok Song, SunYoung Ham, DeokKun Oh, Jintae Hong, and Do-Young Yoon (2014) IL-32θ negatively regulates IL-1β production through its interaction with PKCδ and the inhibition of PU.1 phosphorylation. FEBS Lett. 588: 2822-2829
211. Yun Sun Park, Jeong-Woo Kang, DongHun Lee, ManSub Kim, Yesol Bak, Young Yang, HeeGu Lee, JinTae Hong, Do-Young Yoon (2014) IL-32α modulates promyelocytic leukemia zinc finger gene activity by inhibiting protein kinase Cε-dependent sumoylation. Int J of Biochem & Cell Biol 55: 136-143
210. Yun Sun Park, Jeong-Woo Kang, DongHun Lee, Man Sub Kim, Yesol Bak, Young Yang, Hee Gu Lee, Jin Tae Hong, Do-Young Yoon (2014) IL-32α down-regulates the activity of the B-cell CLL/lymphoma 6 protein by inhibiting protein kinase Cε-dependent SUMO-2 modification. Oncotarget 5(18): 8765-8777
208. Hyun jhung Jhun, Jida Choi, Jaewoo Hong, Siyoung Lee, Areum Kwak, Eunsom Kim, Seunghyun Jo, Soyoon Ryoo, Yoojung Lim, Do-Young Yoon, Jin Tae Hong, Tae Sung Kim, Youngmin Lee, Keeho Song, Soohyun Kim (2014) IL-32 gamma overexpression accelerates streptozotocin (STZ)-induced type 1 diabetes. Cytokine 69: 1-5
207. Yesol Bak, Jeong-Woo Kang, Man Sub Kim, Yun Sun Park, Tae Ho Kwon, Soohyun Kim, Jintae Hong and Do-Young Yoon (2014) IL-32θ downregulates CCL5 expression through its Interaction with PKCδ and STAT3. Cellular Signalling 26, 3007-3015 (2 October 2014)
206. Kang YH, Han SR, Kim JT, Lee SJ, Yeom YI, Min JK, Lee CH, Kim JW, Yoon SR, Yoon DY, Hong KS, Hwang GS, Kim HC, Lee YH, and Lee HG (2014) The EF-hand calcium-binding protein tescalcin is a potential oncotarget in colorectal cancer. Oncotarget 5(8), 2149-2160 (IF = 6.636).
204. Kang JW, Park YS, Kim MS, Lee DH, Bak Y, Ham SY, Song YS, Hong JT, Yoon DY (2014) IL-32α down-regulates β2 integrin (CD18) expression by suppressing PU.1 expression in myeloid cells. Cellular Signalling 26, 1514-1522
203. Jeong-Woo Kang, Yun Sun Park, Dong Hun Lee, Man Sub Kim, Yesol Bak, Sun Young Ham, SuHo Park, Heejong Kim, Joong Hoon Ahn, Jintae Hong and Do-Young Yoon (2014) Interaction network mapping of IL-32 isoforms. Biochimie 101, 248-251
202. Park ES, Yoo JM, Yoo HS, Yoon DY, Yun YP, Hong J (2014) IL-32γ enhances TNFα-induced cell death in colon cancer. Mol Carcinogenesis 53 (suppl 1), E23-35