◎ 세포분화 및 노화연구소 세미나 개최 ◎



▶ 일 시 : 2016. 11. 14(월) 16시

▶ 장 소 : 대학원의학과 세미나실(의학관 5층 3506호실)

▶ 연 자 : 윤재호 박사(National Institutes of Health(NIH) USA)

▶ 주 최 : 세포분화 및 노화연구소 / 생화학교실

▶ 강의제목 : The Novel ephrinB2 binding partner, known as TBC1D24, regulates Neural crest cell migration.

　although Eph-ephrin signaling contributes to the migration of cranial neural crest (CNC) cells, it is still unclear how ephrinB2 transduces signals affecting this event. Using ephrinB2 immunoprecipitation and mass spectrometric analysis, we identified an interaction between ephrinB2 and TBC1d24 that is mediated by Dishevelled. Both ephrinB2 and TBC1d24 morphant embryos display abnormal CNC cell migration, which is rescued by expressing their wild-type counterparts. However, a TBC1d24 mutant that cannot interact with ephrinB2 fails to rescue the TBC1d24 morphant defect. TBC1d24 is known as a GAP for Rab35 that regulates cell-cell adhesion and cell migration through regulating cadherin recycling. Both ephrinB2 and TBC1d24 morphants display increased E-cadherin levels that may disrupt normal CNC migration. In addition, binding of the EphB4 receptor, decreases the interaction between ephrinB2 and TBC1d24, and thus inhibits CNC cell migration. Our results indicate that TBC1d24 is a critical player in ephrinB2 control of CNC cell migration.